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KMID : 1149220080160020219
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Korean Journal of Oriental Medical Prescription
2008 Volume.16 No. 2 p.219 ~ p.228
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The inhibitory effect of Scutellaria baicalensis on type 1 interferon production in Raw 264.7 cells
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Guk Yun-Beom
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Abstract
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Objective : The present study was designed to investigate whether the water extract of the root of Scutellaria baicalensis could regulate lipopolysaccharide (LPS)-induced type 1 interferon.
Methods : To evaluate of type 1 interferon inhibitory effect of the root of Scutellaria baicalensis, we examined type 1 interferon in LPS-stimulated RAW264.7 cells. Furthermore, Interleukin (IL)-10 and interferon regulatory factor (IRF) - 1, 7 expression level were examined to study the inhibition mechanisms.
Results 1. Extract from the root of Scutellaria baicalensis didn’t have any cytotoxity itelf. 2. Extract from the root of Scutellaria baicalensis inhibited interferon-a,b in dose dependant- and type 1 interferon production in time dependant manner. 3. Extract from the root of Scutellaria baicalensis reduced IL-10 and IRF-1, 7 production in LPS-stimulated RAW 264.7 cells.
Conclusion : The extract from the root of Scutellaria baicalensis down-regulated LPS-induced type 1 interferon through suppression of IL-10 and IRF-1, 7 expression. This results suggested that the extract from the root of Scutellaria baicalensis may be a beneficial drug against inflammatory diseases.
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KEYWORD
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Scutellaria baicalensis, lipopolysaccharide (LPS), inflammation, interferon, interleukin - 10, interferon regulatory factor
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